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Parasites of fishes in fresh water
David J. Marcogliese

 
QUALITY ASSESSMENT/QUALITY CONTROL (QA/QC)

  A QA/QC plan is required for any successful monitoring program. It gives results credibility and helps structure the monitoring program. Ways to aid in structuring the program include field notes, sample collection forms, sample processing forms, procedures for verifying taxonomic identifications, data screening, and database management. A sample data sheet is shown in Appendix I. Questionable or uncertain identifications should be verified by an expert. For establishing sampling guidelines to ensure that results are meaningful, see Green (1979) or des Clers (1994). Approximately 20% of program resources should be dedicated to QA/QC.
 
VOLUNTEER AND NON-SPECIALIST INVOLVEMENT

 

People do not require a great deal of training to do adequate dissections to recover parasites. University undergraduate classes can acquire a lot of valuable data. However, people with some expertise or experience are required for many, but not all, identifications.

Volunteers and volunteer networks can contribute cursory observations of large parasites. In fresh water, these include some arthropods on gills and skin of fish. Copepods on gills are sometimes visible with the naked eye; they are white or cream-coloured and often have two egg sacs. The fish louse, Argulus, is quite large, and can be seen crawling on the body surface. Leeches also can be easily seen on the external surface. Certain digenean metacercariae form blackspot and can be seen on the skin or in the flesh of fish. As adults, these parasites infect birds or mammals. The large yellow grub Clinostomum is found in pustule-like capsules just under the skin of fish, often in the head or tail region. The huge, ribbon-like plerocercoids of the cestodes Ligula and Schistocephalus can be found in the body cavities of cyprinids and sticklebacks, respectively. These are tapeworms that use birds as definitive hosts. A large redish nematode, Eustrongylides, also can be seen in the body cavity of fish. This nematode is a larval stage, and its adults infect birds. Other tapeworm plerocercoids (e.g. Diphyllobothrium, Triaenophorus) are clearly visible as cysts on the viscera or in the musculature of fish. Adult cestodes such as Proteocephalus and acanthocephalans can be seen easily if the intestine is cut open.

Non-specialist volunteers should categorize the common macroparasite groups. Flatworms include digeneans (or flukes) and cestodes (or tapeworms). These are flat, and the cestodes are long and segmented, the digeneans short and rounded. Long narrow worm-like organisms are nematodes (or roundworms). Tubular animals with a spiny "head"-like portion are acanthocephalans (or thorny-headed worms). Crustaceans are external parasites with hard body parts. Databases collected by volunteer groups should be maintained independently from those of experts until verification of species is assured.
 

MATERIALS AND SUPPLIERS

 

See sections on other organisms for field sampling and collecting equipment. Standard biological supply houses can provide most of the laboratory materials required. A list of suppliers is provided in Table 2.
 

ACKNOWLEDGEMENTS

 

I am grateful to Dan McLaughlin, Al Shostak, and David Rosenberg, whose comments greatly improved this document.


 
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