Recipes for standard fixatives, preservatives, and stains commonly used in parasitology. After Ash and Orihel (1991).
|
| Material |
Recipe |
| 70% ethanol |
740 mL 95% ethanol + 260 mL water |
| 5% glycerol in 70 % ethanol |
50 mL glycerol + 740 mL 95% ethanol + 210 mL water |
| 10% buffered formalin |
Dissolve 6.10 g dibasic sodium phophate (Na2HPO4) and 0.15 g
monobasic sodium phosphate (NaH2PO4) in 800 mL 37.5%
formaldehyde (=100% formalin) + 7200 mL water (Available
commercially) |
| AFA (alcohol-formalin-acetic
acid) |
100 mL 100% formalin + 500 mL 95% ethanol + 50 mL glacial
acetic acid + 450 mL water |
| Schneider's acetocarmine stain |
45 mL glacial acetic acid + 55 mL water (slowly!). Add 5 g
carmine powder, boil 15 min. Cool and filter. For use, add a few
drops to small dish of 70% ethanol to make solution moderate to
dark pink. (Available commercially) |
| Glycerol-alcohol mixtures for
clearing large nematodes, to be
used sequentially |
1. 50 mL glycerol + 700 mL 95% ethanol + 250 mL water
2. 100 mL glycerol + 700 mL 95% ethanol + 200 mL water
3. 200 mL glycerol + 500 mL 95% ethanol + 300 mL water
4. 500 mL glycerol + 300 mL 95% ethanol + 200 mL water
5. 700 mL glycerol + 100 mL 95% ethanol + 100 mL water
6. 1000 mL glycerol |
| Lactophenol clearing agent |
20 mL glycerol + 10 mL lactic acid + 10 mL melted phenol
crystals + 10 mL water. Mix well. |
| Berland's fluid |
5 mL 100% formalin + 95 mL glacial acetic acid |
